PerfectStart? Green qPCR SuperMix
染料法熒光定量PCR預(yù)混液
目錄號： AQ601-01-V2
單 價：￥440
產(chǎn)品詳情介紹
本產(chǎn)品包含PerfectStart? Taq 熱啟動酶 (利用3種單克隆抗體與Taq DNA Polymerase高效結(jié)合，有效地封閉了DNA聚合酶活性，阻止了低溫下的非特異性擴增)、優(yōu)化的雙陽離子緩沖液、SYBR Green I 熒光染料、dNTPs、PCR增強劑、PCR穩(wěn)定劑。本產(chǎn)
品濃度為2×，使用時只需加入模板、引物、Universal Passive Reference Dye和水，使其工作濃度為1×，即可進行反應(yīng)。
? 3種抗體封閉，特異性高，靈敏度高，擴增效率強，適用物種范圍廣。
? 雙陽離子緩沖液，增強特異性，減少引物二聚體形成，數(shù)據(jù)準(zhǔn)確。
? 配有適用于不同機型的Passive Reference Dye (調(diào)整PCR加樣誤差引起的管間差異 )，數(shù)據(jù)準(zhǔn)確。
擴增效率
以梯度稀釋的質(zhì)粒DNA (10 ng～ 0.1 pg，10倍稀釋)為模板進行擴增得到的擴增曲線和標(biāo)準(zhǔn)曲線。結(jié)果顯示，TransGen產(chǎn)品擴增效率較高，可得到漂亮的擴增曲線和標(biāo)準(zhǔn)曲線。
封閉效果
以不同濃度人gDNA為模板擴增，瓊脂糖凝膠電泳檢測PerfectStart? Taq熱啟動酶封閉效果。結(jié)果顯示，封閉后可顯著提高擴增靈敏度和特異性。
以小麥cDNA為模板，qPCR檢測PerfectStart? Taq熱啟動酶封閉效果。結(jié)果顯示，封閉后可顯著提高擴增特異性。
產(chǎn)品穩(wěn)定性
不同溫度保存及反復(fù)凍融處理后TransGen產(chǎn)品仍可穩(wěn)定擴增
無NTC擴增基因數(shù)量統(tǒng)計
使用TransGen與Company T的產(chǎn)品，擴增58個基因(9個人的基因，7個小鼠的基因，17個水稻的基因，8個煙草的基因，4個擬南芥的基因，10個小麥的基因，3個玉米的基因)。無NTC擴增基因數(shù)統(tǒng)計。結(jié)果顯示，TransGen產(chǎn)品無NTC擴增基因數(shù)高于Company T，擴增效果更佳。
擴增靈敏度
以500 ng的人源RNA反轉(zhuǎn)錄(TransGen, AT311)后得到的cDNA為模板梯度稀釋，分別使用TransGen與Company T產(chǎn)品擴增β-actin (NTC無擴增)。結(jié)果顯示，TransGen產(chǎn)品擴增靈敏度與Company T產(chǎn)品基本一致。
不同物種模板擴增
以不同物種的RNA反轉(zhuǎn)錄(TransGen, AT311)后得到的cDNA為模板分別使用TransGen與Company T的產(chǎn)品進行擴增(NTC無擴增)。結(jié)果顯示，TransGen產(chǎn)品擴增效果與Company T產(chǎn)品基本一致。
產(chǎn)品介紹視頻
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