GelStain
熒光核酸染色試劑
| 目錄號 | 規(guī)格 | 單價 |
|---|---|---|
| GS101-01 | 500 µl | 660 |
| GS101-02 | 1 ml | 1190 |
| GS101-03 | 5×1 ml | 5350 |
產(chǎn)品詳情介紹
GelStain是一種靈敏、穩(wěn)定和相對安全的熒光核酸染色試劑。它可以替代高毒性染色劑－溴化乙錠(EB)，用于瓊脂糖凝膠或聚丙烯酰胺凝膠中dsDNA、ssDNA和RNA的染色。GelStain的靈敏度遠高于EB，并且不需要脫色。GelStain和EB有相同的光譜特性。
特點：
? 無毒性：GelStain獨特的油性和大分子特點使其不能穿透細胞膜進入細胞內(nèi)，艾姆斯氏實驗結(jié)果也表明，該染料的誘變性遠遠小于EB。
? 靈敏度高：適用于不同大小的片段電泳染色，對核酸遷移率的影響小于SYBR Green I。
? 穩(wěn)定性高：適用于使用微波或其它加熱方法制備瓊脂糖凝膠；室溫下在酸性、堿性環(huán)境下極其穩(wěn)定，耐光性強。
? 信噪比高：樣品熒光信號強，背景信號低。
? 操作簡單：與EB一樣，在預(yù)制膠和電泳過程中染料不降解；而電泳后染色過程也只需30分鐘且無需脫色或沖洗，即可直接用紫外凝膠透射儀觀察。
? 適用范圍廣：可選擇電泳前染色（膠染法）或電泳后染色（泡染法）；適用于瓊脂糖凝膠或聚丙烯酰胺凝膠電泳；可用于dsDNA、ssDNA或RNA染色。
? 無需改變?yōu)V光片及觀察裝置：標(biāo)準(zhǔn)的EB濾光片或SYBR濾光片都適用，使用與觀察EB染色相同的普通紫外凝膠透射儀觀察即可，在300 nm紫外光附近可得到最佳激發(fā)。
1.Zhang B, Lin J, Per?ulija V, et al. Structural insights into target DNA recognition and cleavage by the CRISPR-Cas12c1 system[J]. Nucleic Acids Research, 2022.(19.16)
2.Song Y, Jiang W, Afridi S K, et al. Astrocyte-derived CHI3L1 signaling impairs neurogenesis and cognition in the demyelinated hippocampus[J]. Cell Reports, 2024.(IF 7.50)
3.Wang B, Wang M, Zhang H, et al. Canine Adenovirus 1 Isolation Bioinformatics Analysis of the Fiber[J]. Frontiers in Cellular and Infection Microbiology, 2022.(6.07)
4.Zheng K, Cai Y, Chen W, et al. Development, identification, and application of a germplasm specific SCAR Marker for Dendrobium officinale Kimura et Migo[J]. Frontiers in Plant Science, 2021.(5.75)
5.Fu J, Yao F, An Y, et al. Novel bispecific aptamer targeting PD-1 and nucleolin for cancer immunotherapy[J]. Cancer Nanotechnology, 2023.(5.70)
6.Zhu J, Li J, Ma D, et al. SDH mutations confer complex cross-resistance patterns to SDHIs in Corynespora cassiicola[J]. Pesticide Biochemistry and Physiology, 2022.(4.97)
7.Sun R, Zhang X, Gong T, et al. Knockdown H19 Accelerated iPSCs Reprogramming through Epigenetic Modifications and Mesenchymal-to-Epithelial Transition[J]. Biomolecules, 2024.(4.80)
8.Zhang S Y, Yan Q, Zhao J, et al. Distinct multitrophic biodiversity composition and community organization in a freshwater lake and a hypersaline lake on the Tibetan Plateau[J]. Iscience, 2024.(IF 4.60)
9.Sun X, Wang Z, Cong X, et al. Mitochondrial gene COX2 methylation and downregulation is a biomarker of aging in heart mesenchymal stem cells[J]. International Journal of Molecular Medicine, 2021.(4.10)
